Journal: Frontiers in stroke
Article Title: Compromised endothelial progenitor cell exosomal communication with endothelial cells in hypertension ischemia conditions
doi: 10.3389/fstro.2022.1015463
Figure Lengend Snippet: EPC-EXs derived from R+ mice are less effective on rescuing ECs from Ang II plus H/R-induced apoptosis and compromised cell viability. (A) representative plots, and summarized data of cell apoptosis. (B) cleaved cas-3/β-actin expression in ECs co-cultured with the two types of EPC-EXs. (C) cell viability of ECs co-cultured with the two types of EPC-EXs. * p < 0.05, vs. control; + p < 0.05, vs. vehicle; $ p < 0.05, vs. WT-EPC-EXs. Data expressed as mean ± SEM, n = 6/group. EC+AngII+H/R: Mouse brain microvascular ECs (Cell Biologics) subjected to Ang II (10–6 M) plus hypoxia/reoxygenation (H/R) injury. Primary EC+H/R: primary brain microvascular ECs from R+ mice subjected to H/R injury. Control cells were cultured in normal conditions.
Article Snippet: A mouse brain microvascular ECs (Cell Biologics, Inc; Cat # C57–6023) were used in this study.
Techniques: Derivative Assay, Expressing, Cell Culture, Control